Sow

The seeds are collected during the fruit ripening period from August to September every year. The well-growing golden plum is selected for picking. The seeds are kneaded after the capsule is dried, and the pure seeds obtained after air drying are used for standby. Generally stored in bags at low temperature, soaking seeds with warm water for 1-2 days before sowing can significantly improve the germination rate of golden plum seeds. It is required to plough deeply, rake carefully, and apply sufficient base fertilizer to make a flat border 1.0-1.5m wide for sowing

Sowing can be carried out in early spring or in late autumn without covering the soil. Sowing can be carried out in about 3-4 days after heavy rain. Sowing can be carried out in spring without covering the soil. Sowing can be carried out in about 3-4 days after heavy rain. Sowing can be carried out in spring without covering the soil. Sowing can be carried out in about 3-4 days after heavy rain

Cutting

Generally, it is carried out in spring or autumn. Select the robust 1-2-year-old branches, cut them into cuttings 10-15 cm long, the cutting mouth is flat and oblique, and soak them with auxin or rooting powder. The seedling bed of Prunus mume should be watered with water, and cuttings can be carried out when the water falls behind, with a row spacing of 10cm × 10 cm, the cutting depth is 2 / 3 of the cutting ear length

Ramet

Ramet propagation is generally carried out during cultivation in spring or autumn. Ramets must carry soil balls, which is conducive to survival. The root system of Prunus mume can be wrapped in nylon bags or directly planted in flower pots. Try not to damage the root system of Prunus mume. While digging, they are packaged. After digging and packaging, they are transported in time and watered frequently, which is conducive to survival

Tissue culture

Materials obtained: select the young branches of golden plum, wash them in running water, cut them into 3cm long, absorb the excess water with filter paper, soak them in 70% alcohol for 20 seconds on the super clean workbench, rinse them with sterile water for 3 times, transfer them into 0.1% mercuric solution, shake them for 5 minutes, rinse them with bacteria free water for 5 times, and then put them into a small disk covered with filter paper after high-pressure sterilization, and cut them into a small stem segment with a bud of 0.5-1.0 cm, Inoculation was cultured in differentiation medium

Cluster bud induction: the aseptic small stem segment gradually differentiated into cluster buds at the stem nodes after 20 days on the differentiation medium, and the buds were 1-2.5 cm long after 30 days. At this time, the obtained clustered bud stems are cut off, cut into small segments of about 1 cm, and transferred to the propagation medium for culture. At 40 days, the average seedling height is 3-4 cm, and the proliferation coefficient is 6-8 times

Rooting and Cultivation: when the buds and seedlings of Prunus mume grow to 2-3cm high, cut off the tender stems and transfer them to the rooting medium. After 2 weeks, each plant has 3-5 roots, and the rooting rate is 99%. Put the rooted test tube seedlings in the greenhouse without opening the cover for 5 days, open the cover for 2 days, take them out and wash them, soak them in clean water for 2-3 hours, transplant them into the matrix of vermiculite, river sand and forest soil (5:1:3), cover them with plastic film, put up a shading net to prevent direct sunlight, and keep the humidity at about 90%. Remove the plastic film after 10 days, and the new roots and leaves begin to develop, with a survival rate of 93.6%