Ramet propagation of Cartland

The operation of ramet method is relatively simple, which can be used in general families. It is suitable to separate plants in spring and autumn and rainy season. Use a sharp cutting tool to cut between the two "blue head" footings, carefully cut the rhizome at the base, and disinfect the incision. Each clump needs at least 2 pseudobulbs with new buds

Aseptic seeding and tissue culture of Cartland

In the early stage, it is mainly liquid culture to promote the rapid germination of seeds, and then the protocorm can be directly inoculated on the culture medium to realize one-step seedling formation. The specific steps are as follows:

Materials

When flowering, the healthy mother plant of Cartland is selected for self pollination and hybridization. The fruit can be basically mature about 120-150 days after pollination. 　　　　

Aseptic seeding

Select the mature fruit, soak the fruit with 75% alcohol for half a minute, then disinfect it, put the fruit into 0.1% mercuric chloride solution, soak it for 20 minutes, rinse it with sterile water for 4 or 5 times, cut the fruit, inoculate the white powdered seed into the seed germination liquid medium, and shake it at 150 rpm. The embryo germinated after one week and formed a green protocorm with a diameter of 1-2mm after 20 days. 　　

Seedling culture

The protocorm formed can be inoculated on the seedling culture medium with straw, and a complete small plant can be formed in one month. After continuous culture for 45 days, it can grow into strong seedlings. 　　　

Test tube seedling transplanting

When the test tube plantlets can grow to 3,4 cm high, transfer to the natural light to refine the plantlets for 1-2 weeks, and observe the formation of a better cuticle. After taking it out of the glass bottle, wash the culture medium of the root, move it into the cornerstone of the mixing pond and the carbonized bark matrix, maintain appropriate humidity and ventilation< span>